International Journal of Scientific Research and Engineering Development

International Journal of Scientific Research and Engineering Development

( International Peer Reviewed Open Access Journal ) ISSN [ Online ] : 2581 - 7175

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Phytochemical Screening, Antimicrobial and Antioxidant Activities of Celtis integrifolia (Lam.) Leaves

    International Journal of Scientific Research and Engineering Development (IJSRED)

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Published Issue : Volume-4 Issue-2
Year of Publication : 2021
Unique Identification Number : IJSRED-V4I2P3
Authors : Sa’id,Abba Idris, Abba,Halima Mohammed
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Abstract :

Assessment of phytochemical composition, antimicrobial and antioxidant activities of the methanolic leaves extract of C. integrifolia (Lam.) was performed. The screening for phytoconstituents was achieved using standard methods revealed the presence of Alkaloids, Saponins, Phenols, Terpenoids, Steroids, Tannins, Flavonoids, and Glycosides while Anthraquinones was absent. Alkaloids had (19.7%), Saponins (107.78mg DE/g), Phenols (22.83mg GAE/g), Terpenoids (45.8%), Steroids (664.3µg/ml), Tannins (270.56mg TAE/g), Flavonoids (487.33mg QE/g) and Cardiac Glycosides (10.2%). Microbes used were subjected to biochemical tests for proper identification for clinical isolates of Salmonella typhi, Staphylococcus aureus, Escherichia coli, Shigella spp.Agar well diffusion method was used at concentrations; 500mg/ml, 250mg/ml, 125mg/ml 62.5mg/ml. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extract ranges from 250-500mg/ml:Salmonella typhi, 125-500 mg/ml:Staphylococcus aureus, 250 -500mg/ml:Escherichia coli and 125-250 mg/ml: Shigella spp. 125-250 mg/ml respectively. The extract showed significant inhibition against all the test organisms, hence bactericidal in its effects. The Ferric-reducing antioxidant power (FRAP) of the leaf extract showed significant activity with 43.11mgAAE/g. The half maximal activity concentration (AC50) was 10.87mg/ml and 9.43mg/ml for ascorbic acid standard. This study concludes that the leaf extract of C. integrifolia is related to recent findings that led to the identification of compounds having pharmacological effects such as antimicrobial, anti-inflammatory and antioxidant properties. Thus, C. integrifolia holds bioactive components that have extensive spectrum of pharmacological properties that can be used as a good source of drugs to manage typhoid, bleeding and dysentery.