International Journal of Scientific Research and Engineering Development

Vibrio parahaemoliyticusrelated food poisoning has been increasing recently. These bacteria in seawater and aquatic products can cause gastroenteritis during consumption. The outbreaks of V. parahaemolyticus, especially in coastal regions, need a rapid and sensitive detection method. There are several limitations to the traditional methods of identifying harmful microorganisms. Some limitations are long growing seasons, risk of cross-contamination, reliance on complicated apparatus, and expensive reagents. To address these difficulties. A rapid, accurate, and simple method for identifying V. parahaemolyticus becomes increasingly essential. This study aimed to develop a novel aptamer-based sensor that is rapid, susceptible, compact, and specific. One approach uses fluorescence resonance energy transfer (FRET) and graphene oxide. In this method, V. parahaemolyticus causes the aptamer to detach from the graphene oxide surface, which leads to fluorescence recovery and the ability to calculate bacterial concentration using fluorescence intensities. However, this method only detects large quantities of V. parahaemolyticus and is unresponsive to low amounts. Despite these drawbacks, the approach showed great specificity for Vibrio parahaemolyticus compared to other foodborne harmful microorganisms. It can identify Vibrio parahaemolyticus at quantities of 10 29 5 CFU/mL or more, indicating potential for assuring food safety, although additional development is required. This study opened the door to developing fast, sensitive, and inexpensive technologies for identifying foodborne infections.